Article
Point mutation of a conserved arginine (104) to lysine introduces hypersensitivity to inhibition by glyphosate in the 5-enolpyruvylshikimate-3-phosphate synthase of Bacillus subtilis.
FEBS letters - 30 Oct 1995
Selvapandiyan A, Majumder K, Fattah F A, Ahmad S, Arora N, Bhatnagar R K
Abstract excerpt
The role of a conserved arginine (R104) in the putative phosphoenol pyruvate binding region of 5-enolpyruvyl shikimate-3-phosphate synthase of Bacillus subtilis has been investigated. Employing site directed mutagenesis arginine was substituted by lysine or glutamine. Native and mutant proteins were expressed and purified to near homogeneity. Estimation of Michaelis and inhibitor constants of the native and...
Topics
- 3-Phosphoshikimate 1-Carboxyvinyltransferase
- Alkyl and Aryl Transferases
- Amino Acid Sequence
- Arginine
- Bacillus subtilis
- Binding Sites
- Conserved Sequence
- Enzyme Inhibitors
- Glutamine
- Glycine