Article
Chicken monoclonal antibody isolated by a phage display system
1 Aug 1996
Abstract excerpt
A method for obtaining chicken mAb using a phage display system was established. Chickens were hyperimmunized with murine serum albumin, and polyadenylated RNA was extracted from their spleen cells. cDNA of V regions of heavy or lambda-chain genes were obtained by reverse transcription-PCR using a pair of newly designed primers. A phage display library of 1.4 x10(7) clones expressing the chicken Ab variable...
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