Article
Gain of D-alanyl-D-lactate or D-lactyl-D-alanine synthetase activities in three active-site mutants of the Escherichia coli D-alanyl-D-alanine ligase B.
Biochemistry - 13 Aug 1996
Park I S, Lin C H, Walsh C T
Abstract excerpt
Escherichia coli D-Ala-D-Ala ligase (Ddl) and the vancomycin resistance-conferring protein VanA are homologues, but VanA has gained the ability to activate D-lactate (D-Lac) and make the depsipeptide D-Ala-D-Lac as well as D-Ala-D-Ala. This depsipeptide ligase activity of VanA is its crucial cata...
Topics
- Alanine
- Anti-Bacterial Agents
- Bacterial Proteins
- Binding Sites
- Carbon-Oxygen Ligases
- Drug Resistance
- Escherichia coli
- Hydrogen-Ion Concentration
- Lactates
- Ligases
- Mutation
- Peptide Synthases
- Substrate Specificity
- Vancomycin