Article
Point mutation analysis of archived cytogenetic slide DNA.
Cytogenetics and cell genetics - 1 Jan 1996
Sago H, Goldberg J D, Lebo R V
Abstract excerpt
Archived Giemsa-stained cytogenetic slide repositories represent valuable DNA resources for medical, scientific, and forensic studies. Sequencing readily identified a Charcot-Marie-Tooth disease point mutation in a 209-bp PCR amplified product. With optimal PCR primers and amplification conditions, our protocol quickly and reliably isolated sufficient DNA for at least 12 independent PCR amplification reactions...
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