Effect of salt concentration on the pKa of acidic residues in lysozyme.

doi:10.1093/jb/118.5.946

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Article

Effect of salt concentration on the pKa of acidic residues in lysozyme.

Journal of biochemistry - 1 Nov 1995

Abe Y, Ueda T, Iwashita H, Hashimoto Y, Motoshima H, Tanaka Y, Imoto T

Abstract excerpt

We determined the pKa values of acidic residues in hen lysozyme by comparing the pH dependency of stability between wild type and mutant lysozymes in which a negative charge is eliminated. In the comparison of the stability between wild type and a mutant lysozyme, the difference in pH titration c...

Topics

Amino Acids
Base Sequence
Enzyme Stability
Guanidine
Guanidines
Kinetics
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Muramidase
Mutation
Protein Denaturation
Recombinant Proteins
Saccharomyces cerevisiae
Sodium Chloride
Thermodynamics

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