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[Analysis of the effect of replacing Lys(-20) in the alkaline phosphatase signal peptide on secretion of this enzyme].

Biokhimiia (Moscow, Russia) - 1 Apr 1996

Karamysheva Z N, Karamyshev A L, Ksenzenko V N, Nesmeianova M A

Abstract excerpt

The effect of substitutions for the positively charged Lys(-20) in the N-terminal domain of the E. coli alkaline phosphatase signal peptide on enzyme secretion has been studied. Mutant alkaline phosphatases were obtained by the amber-suppressor method. An amber mutation was introduced in the appropriate position of the alkaline phosphatase gene using oligonucleotide-directed mutagenesis. This was followed by...

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PMID: 8724791

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[Analysis of the effect of replacing Lys(-20) in the alkaline phosphatase signal peptide on secretion of this enzyme].PMID 8724791Read ↗

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