Article
[Biogenesis and secretion of alkaline phosphatase and its mutants in Escherichia coli. III. Substitution of N-terminal amino acids of alkaline phosphatase affect its biogenesis].
Molekuliarnaia biologiia - 1 Jan 2000
Karamyshev A L, Kalinin A E, Khmel'nitskiĭ M I, Shliapnikov M G, Ksenzenko V N, Nesmeianova M A
Abstract excerpt
The effect of the N-terminal amino acid substitution on E. coli alkaline phosphatase biogenesis has been studied. The substitutions of Ser, Gln, Tyr, Leu, Gly, Ala, Glu, Phe, His, Cys, Lys and Pro for Arg(+1) were obtained by creating amber mutation at the corresponding position within phoA gene and expressing this mutated gene in E. coli strains that produce the amber-suppressor tRNAs. All mutant proteins were...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Amino Acids
- Base Sequence
- Enzyme Precursors
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Processing, Post-Translational