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Article

Characterization of manganese(II) binding site mutants of manganese peroxidase.

Biochemistry - 9 Jul 1996

Kishi K, Kusters-van Someren M, Mayfield M B, Sun J, Loehr T M, Gold M H

Abstract excerpt

A series of site-directed mutants, E35Q, E39Q, and E35Q-D179N, in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium, was created by overlap extension, using the polymerase chain reaction. The mutant genes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. The mutant manganese...

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PMID: 8688436
DOI: 10.1021/bi960679c

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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