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Article

Engineering of the H2O2-binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2.

FEBS letters - 30 Nov 2001

Miyazaki C, Takahashi H

Abstract excerpt

The manganese peroxidase produced by Phanerochaete chrysosporium, which catalyzes the oxidation of Mn(2+) to Mn(3+), is easily inactivated by the hydrogen peroxide (H2O2) presented in the reaction. We attempted to increase H2O2 resistance by the conformational stabilization around the H2O2-binding pocket. Based on its structural model, engineering of oxidizable Met273 located near the pocket to a non-oxidizable...

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Identifiers

PMID: 11734216
DOI: 10.1016/s0014-5793(01)03127-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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