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Article

Site-directed mutations at phenylalanine-190 of manganese peroxidase: effects on stability, function, and coordination.

Biochemistry - 8 Apr 1997

Kishi K, Hildebrand D P, Kusters-van Someren M, Gettemy J, Mauk A G, Gold M H

Abstract excerpt

A series of site-directed mutants, F190Y, F190L, F190I, and F190A, in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium was generated by overlap extension with the polymerase chain reaction. The mutant genes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. The manganese peroxidase...

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Identifiers

PMID: 9100022
DOI: 10.1021/bi962627t

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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