Article
Site-directed mutations at phenylalanine-190 of manganese peroxidase: effects on stability, function, and coordination.
Biochemistry - 8 Apr 1997
Kishi K, Hildebrand D P, Kusters-van Someren M, Gettemy J, Mauk A G, Gold M H
Abstract excerpt
A series of site-directed mutants, F190Y, F190L, F190I, and F190A, in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium was generated by overlap extension with the polymerase chain reaction. The mutant genes were expressed in P. chrysosporium during primary...
Topics
- Basidiomycota
- Circular Dichroism
- Electron Spin Resonance Spectroscopy
- Enzyme Stability
- Ferrocyanides
- Heme
- Hydrogen-Ion Concentration
- Isoenzymes
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction
- Peroxidases
- Phenylalanine
- Polymerase Chain Reaction
- Protein Denaturation
- Spectrophotometry
- Temperature