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Article

Alternative model for mechanism-based inhibition of Escherichia coli ribonucleotide reductase by 2'-azido-2'-deoxyuridine 5'-diphosphate.

Biochemistry - 30 Nov 1993

Salowe S, Bollinger J M, Ator M, Stubbe J, McCraken J, Peisach J, Samano M C, Robins M J

Abstract excerpt

Ribonucleotide reductase (RDPR) from Escherichia coli is composed of two subunits, R1 and R2, and catalyzes the conversion of nucleotides to deoxynucleotides. The mechanism of inactivation of RDPR by 2'-azido-2'-deoxynucleoside 5'-diphosphate (N3UDP) has been examined using a variety of isotopically labeled derivatives: (1'-, 2'-, 3'-, or 4'-[2H])-N3UDPs and 2'-[15N3, 13C]-N3UDP. Electron paramagnetic resonance...

Topics

Azides
Cysteine
Deoxyuracil Nucleotides
Deuterium
Electron Spin Resonance Spectroscopy
Escherichia coli
Free Radicals
Models, Chemical
Mutation
Nitrogen
Ribonucleotide Reductases

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Tyrosine

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Identifiers

PMID: 8251496
DOI: 10.1021/bi00210a026

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8251496-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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