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Article

Transition-state structures for the native dual-specific phosphatase VHR and D92N and S131A mutants. Contributions to the driving force for catalysis.

Biochemistry - 4 Jun 1996

Hengge A C, Denu J M, Dixon J E

Abstract excerpt

Isotope effects have been measured for the reaction of the human dual-specific phosphatase VHR with p-nitrophenyl phosphate (pNPP). Isotope effects in the nonbridge oxygen atoms, in the bridge oxygen atom, and in the nitrogen atom were measured by the competitive method using an isotope ratio mass spectrometer. These are isotope effects on V/K, and give information on the chemical step of phosphoryl transfer from...

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PMID: 8679534
DOI: 10.1021/bi960255i

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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