Anomeric specificity of the native and mutant forms of human beta-cell glucokinase.

Abstract excerpt

The anomeric specificity of wild-type human beta-cell glucokinase and six of its mutant forms toward alpha- and beta-D-glucose was examined over 6-min incubation at 30 degrees C. When D-[U-14C]glucose at anomeric equilibrium was used as substrate, the wild-type form yielded a maximal velocity of 76 U/mg, a K(m) of 4-5 mM, and a Hill coefficient close to 1.2. The maximal velocity (2 to 89 U/mg) and K(m) (2.4 to...

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