Article
A single amino acid change in Escherichia coli glycerol kinase abolishes glucose control of glycerol utilization in vivo.
Journal of bacteriology - 1 May 1996
Pettigrew D W, Liu W Z, Holmes C, Meadow N D, Roseman S
Abstract excerpt
Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in glucose control of glycerol metabolism. Its catalytic activity is inhibited allosterically by the glycolytic intermediate, fructose 1,6-biphosphate, and by the phosphotransferase system phosphoca...
Topics
- Allosteric Regulation
- Cloning, Molecular
- Dose-Response Relationship, Drug
- Escherichia coli
- Fructosediphosphates
- Glucose
- Glycerol
- Glycerol Kinase
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Sequence Analysis, DNA
- Species Specificity