Article
The conserved Lys-95 charged residue cluster is critical for the homodimerization and enzyme activity of human ribonucleotide reductase small subunit M2.
The Journal of biological chemistry - 10 Jan 2014
Chen Xinhuan, Xu Zhijian, Zhang Lingna, Liu Hongchuan, Liu Xia, Lou Meng, Zhu Lijun, Huang Bingding, Yang Cai-Guang, Zhu Weiliang, Shao Jimin
Abstract excerpt
Ribonucleotide reductase (RR) catalyzes the reduction of ribonucleotides to deoxyribonucleotides for DNA synthesis. Human RR small subunit M2 exists in a homodimer form. However, the importance of the dimer form to the enzyme and the related mechanism remain unclear. In this study, we tried to identify the interfacial residues that may mediate the assembly of M2 homodimer by computational alanine scanning based...
Topics
- Amino Acid Substitution
- Biocatalysis
- Cell Proliferation
- Crystallography, X-Ray
- Electron Spin Resonance Spectroscopy
- G1 Phase Cell Cycle Checkpoints
- Glutamic Acid
- Green Fluorescent Proteins
- HEK293 Cells