Article
Isolation and characterization of porcine Müller cells. Myofibroblastic dedifferentiation in culture.
Investigative ophthalmology & visual science - 1 Apr 1996
Guidry C
Abstract excerpt
PURPOSE: To characterize phenotypic and antigenic changes in isolated Müller cells during proliferation in extended culture. METHODS: Müller cells were isolated from porcine retina by sequential papain and DNase digestion, trituration, and density gradient centrifugation. The identity of the isolated cells was confirmed by immunodetection of carbonic anhydrase II (CA-II), cellular retinaldehyde-binding protein...
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