Article
Expression of a human mutant monocyte chemotactic protein 3 in Pichia pastoris and characterization as an MCP-3 receptor antagonist.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research - 1 Nov 1995
Masure S, Paemen L, Proost P, Van Damme J, Opdenakker G
Abstract excerpt
The cDNA encoding human monocyte chemotactic protein 3 (hMCP-3) was cloned in pHIL-S1, a vector designed for inducible secreted heterologous expression in the methylotrophic yeast Pichia pastoris. After transformation of P. pastoris by electroporation, several clones with the human MCP-3 gene int...
Topics
- Amino Acid Sequence
- Base Sequence
- Chemokine CCL7
- Cloning, Molecular
- Cytokines
- DNA, Complementary
- Humans
- Molecular Sequence Data
- Monocyte Chemoattractant Proteins
- Mutation
- Pichia
- Receptors, Cytokine
- Recombinant Proteins