Article
Expression of a human mutant monocyte chemotactic protein 3 in Pichia pastoris and characterization as an MCP-3 receptor antagonist.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research - 1 Nov 1995
Masure S, Paemen L, Proost P, Van Damme J, Opdenakker G
Abstract excerpt
The cDNA encoding human monocyte chemotactic protein 3 (hMCP-3) was cloned in pHIL-S1, a vector designed for inducible secreted heterologous expression in the methylotrophic yeast Pichia pastoris. After transformation of P. pastoris by electroporation, several clones with the human MCP-3 gene integrated at the alcohol oxidase (AOX-1) locus were isolated. One of these clones (M30) expressed the mature MCP-3...
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