High-throughput genotyping using horizontal polyacrylamide gels with wells arranged for microplate array diagonal gel electrophoresis (MADGE).

Article

High-throughput genotyping using horizontal polyacrylamide gels with wells arranged for microplate array diagonal gel electrophoresis (MADGE).

BioTechniques - 1 Nov 1995

Day I N, Humphries S E, Richards S, Norton D, Reid M

Abstract excerpt

Genotyping (typing of genetic variation) typically involves PCR followed by an allele-specific oligonucleotide-binding assay, restriction enzyme digest or direct check of the outcome of a PCR designed to distinguish genotype. Electrophoresis can resolve "bound" from "free" oligonucleotide, as wel...

Topics

Electrophoresis, Polyacrylamide Gel
Ethidium
Genotype
Polymerase Chain Reaction

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