Higher resolution microplate array diagonal gel electrophoresis: application to a multiallelic minisatellite.

doi:10.1002/1098-1004(200006)15:6<565::AID-HUMU8>3.0.CO;2-7

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Article

Higher resolution microplate array diagonal gel electrophoresis: application to a multiallelic minisatellite.

Human mutation - 1 Jan 2000

O'Dell S D, Chen X, Day I N

Abstract excerpt

The 5' polymorphic region of the insulin (INS, MIM# 176730) gene contains a variable tandem repetition of 14-15 bp (a variable number of tandem repeats (VNTR) locus). After PCR amplification, we achieved precise sizing of class I alleles (range 641 to 843 bp) on 96-well open-face polyacrylamide microplate array diagonal gel electrophoresis (MADGE) gels, obtaining resolution of the 2% mobility difference which...

Topics

Alleles
Electrophoresis, Polyacrylamide Gel
Genes, MHC Class I
Genotype
Humans
Insulin
Male
Middle Aged
Minisatellite Repeats
Nucleic Acid Conformation
Polymerase Chain Reaction
Sequence Analysis, DNA
Spectrometry, Fluorescence

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Identifiers

PMID: 10862086
DOI: 10.1002/1098-1004(200006)15:6<565::AID-HUMU8>3.0.CO;2-7

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