Article
Overexpression of MerT, the mercuric ion transport protein of transposon Tn501, and genetic selection of mercury hypersensitivity mutations.
Molecular & general genetics : MGG - 15 Jan 1996
Hobman J L, Brown N L
Abstract excerpt
The small (116 amino acids) inner membrane protein MerT encoded by the transposon Tn501 has been overexpressed under the control of the bacteriophage T7 expression system. Random mutants of MerT were made and screened for loss of mercuric ion hypersensitivity. Several mutant merT genes were selected and sequenced: Cys24Arg and Cys25Tyr mutations abolish mercury resistance, as do charge-substitution mutations in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.