Article
Selection and characterization of mercury-independent activation mutants of the Tn501 transcriptional regulator, MerR.
Microbiology (Reading, England) - 1 Oct 1998
Parkhill Julian, Lawley Blair, Hobman Jonathan L, Brown Nigel L
Abstract excerpt
MerR is the transcriptional regulator of the mercury-resistance (mer) operon of transposon Tn501, acting at the mer promoter as both an activator in the presence of mercuric salts and a repressor in their absence. This paper reports a method for selection of constitutive activator mutants, which...
Topics
- Bacterial Proteins
- Binding Sites
- Cysteine
- DNA Transposable Elements
- DNA, Bacterial
- DNA-Binding Proteins
- Drug Resistance, Microbial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Markers
- Helix-Turn-Helix Motifs
- Mercury
- Mutation
- Phenotype
- Repressor Proteins
- Response Elements
- Thermodynamics
- Trans-Activators