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Article

Phage transposon mutagenesis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Siegrist M Sloan, Rubin Eric J

Abstract excerpt

Phage transduction is an attractive method of genetic manipulation in mycobacteria. PhiMycoMarT7 is well suited for transposon mutagenesis as it is temperature sensitive for replication and contains T7 promoters that promote transcription, a highly active transposase gene, and an Escherichia coli oriR6 K origin of replication. Mycobacterial transposon mutant libraries produced by PhiMycoMarT7 transduction are...

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PMID: 20560067
DOI: 10.1007/978-1-59745-207-6_21

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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