Article
Detection of altered protein conformations in living cells.
Journal of molecular biology - 26 Jan 2001
Raquet X, Eckert J H, Müller S, Johnsson N
Abstract excerpt
The maturation, conformational stability, and the rate of in vivo degradation are specific for each protein and depend on both the intrinsic features of the protein and those of the surrounding cellular environment. While synthesis and degradation can be measured in living cells, stability and maturation of proteins are more difficult to quantify. We developed the split-ubiquitin method into a tool for detecting...
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