Article
Mechanism of interferon action. Biochemical and genetic evidence for the intermolecular association of the RNA-dependent protein kinase PKR from human cells.
Virology - 1 Jan 1996
Ortega L G, McCotter M D, Henry G L, McCormack S J, Thomis D C, Samuel C E
Abstract excerpt
The interferon-inducible protein kinase (PKR) is activated by an RNA-dependent autophosphorylation. Structure-function studies of the 551 amino acid PKR kinase from human cells have revealed that catalytic-deficient PKR mutants such as PKR(1-551)K296R display a dominant negative behavior when exp...
Topics
- Animals
- Cell Line
- Genes, Reporter
- Haplorhini
- Histidine
- Humans
- Interferons
- Mutation
- Phosphorylation
- Protein Serine-Threonine Kinases
- RNA
- Reoviridae
- Transfection
- eIF-2 Kinase