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Translation of Sindbis virus mRNA: analysis of sequences downstream of the initiating AUG codon that enhance translation

1 Feb 1996

Abstract excerpt

Alphaviruses, particularly Sinbis virus and Semliki Forest virus, are proving to be useful vectors for the expression of heterologous genes. In infected cells, these self-replicating vectors (replicons) transcribe a subgenomic mRNA that codes for a heterologous protein instead of the structural proteins. We reported recently that translation of the reporter gene lacZ is enhanced 10-fold when the coding sequences...

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PMID: 8551579
DOI: 10.1128/jvi.70.2.1182-1190.1996

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