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Rolling circle DNA replication by extracts of herpes simplex virus type 1-infected human cells

1 Feb 1996

Abstract excerpt

Whole-cell extracts of herpes simplex virus type 1-infected human cells (293 cells) can promote the rolling circle replication of circular duplex DNA molecules. The products of the reaction are longer than monomer unit length and are the result of semiconservative DNA replication by the following criteria: (i) resistance to DpnI and susceptibility to MboI restriction enzymes, (ii) shift in density on a CsCl...

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PMID: 8551573
DOI: 10.1128/jvi.70.2.1132-1136.1996

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