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Rolling circle replication of human papillomavirus type 16 DNA in epithelial cell extracts

9 Nov 2010

Abstract excerpt

Replication of human papillomavirus (HPV) genomes requires an origin of replication and two viral proteins: the DNA helicase E1 and the auxiliary factor E2. To dissect the profile of HPV replication in the epithelium, we analyzed replication of an HPV16 origin-containing plasmid in human epithelial cell extracts supplemented with purified E1 and E2. We found that in addition to well-defined circular replication...

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PMID: 21059156
DOI: 10.1111/j.1365-2443.2010.01458.x

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