Article
Identification of phosphorylation sites in the mouse oestrogen receptor.
The Journal of steroid biochemistry and molecular biology - 1 Dec 1995
Lahooti H, White R, Hoare S A, Rahman D, Pappin D J, Parker M G
Abstract excerpt
Phosphorylation sites in the mouse oestrogen receptor, expressed in COS-1 cells in the presence of 17 beta-oestradiol, have been mapped by solid phase microsequencing. The receptor was first radio-labelled with [32P]orthophosphate and a number of 3H- or 14C-labelled amino acids, immunopurified and then tryptic peptides were separated by thin layer chromatography or high performance liquid chromatography. Amino...
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