Article
Phosphorylation of transfected wild type and mutated progesterone receptors.
The Journal of biological chemistry - 25 Sept 1991
Chauchereau A, Loosfelt H, Milgrom E
Abstract excerpt
An expression vector encoding wild type or mutated forms of the rabbit progesterone receptor was transfected into COS-7 cells and phosphorylation was studied by incubation with 32Pi followed by specific immunoprecipitation. The features of phosphorylation of the wild type receptor were identical to those previously observed in uterine cells: there was a basal level of phosphorylation which was increased...
Topics
- Animals
- Cell Line
- Cell Nucleus
- Cyanogen Bromide
- DNA
- Down-Regulation
- Electrophoresis, Polyacrylamide Gel
- Hydroxylamine
- Hydroxylamines
- Mutation
- Phosphorylation
- Precipitin Tests