Kinetic models for unfolding and refolding of ribonuclease T1 with substitution of cis-proline 39 by alanine.

doi:10.1006/jmbi.1993.1337

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Article

Kinetic models for unfolding and refolding of ribonuclease T1 with substitution of cis-proline 39 by alanine.

Journal of molecular biology - 5 Jun 1993

Mayr L M, Schmid F X

Abstract excerpt

The replacement of cis proline 39 of ribonuclease T1 by an alanine residue leads to a decrease in stability by about 20 kJ/mol and to major changes in the folding kinetics that are not easily explained by the proline model for protein folding. In particular, a novel very slow reaction is observed...

Topics

Alanine
Amino Acid Isomerases
Carrier Proteins
Catalysis
Circular Dichroism
Humans
Hydrogen-Ion Concentration
Kinetics
Models, Chemical
Mutation
Peptidylprolyl Isomerase
Proline
Protein Denaturation
Protein Folding
Recombinant Proteins
Ribonuclease T1

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