Article
The rate of isomerisation of peptidyl-proline bonds as a probe for interactions in the physiological denatured state of chymotrypsin inhibitor 2.
Journal of molecular biology - 20 Jun 1997
Tan Y J, Oliveberg M, Otzen D E, Fersht A R
Abstract excerpt
There are four peptidyl-proline bonds in the 64-residue protein chymotrypsin inhibitor 2 (CI2), all of which are in the trans conformation in the native structure. The isomerisation of one or more of these peptidyl-proline bonds to the cis conformation in the denatured state gives rise to heterog...
Topics
- Amino Acid Isomerases
- Carrier Proteins
- Catalysis
- Chymotrypsin
- Circular Dichroism
- Hydrogen-Ion Concentration
- Isomerism
- Kinetics
- Mutation
- Peptides
- Peptidylprolyl Isomerase
- Plant Proteins
- Proline
- Protein Denaturation
- Protein Folding
- Serine Proteinase Inhibitors
- Spectrometry, Fluorescence
- Temperature