Article
Cloning and sequencing of a gene involved in the synthesis of the capsular polysaccharide of Streptococcus pneumoniae type 3.
Molecular & general genetics : MGG - 1 May 1993
García E, García P, López R
Abstract excerpt
A 4.5 kb ScaI chromosomal DNA fragment of a clinical isolate of Streptococcus pneumoniae serotype 3 was cloned in Escherichia coli. Combined genetic and molecular analyses have allowed the localization, in a 781 bp EcoRV subfragment, of a gene (cap3-1) directly responsible for the transformation...
Topics
- Amino Acid Sequence
- Bacterial Capsules
- Bacterial Proteins
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Phenotype
- Polysaccharides, Bacterial
- Restriction Mapping
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Streptococcus pneumoniae
- Transformation, Genetic
- Uridine Diphosphate Glucose Dehydrogenase