Article
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene - 1 Jan 2000
Myoda T T, Lowther S V, Funanage V L, Young F E
Abstract excerpt
The structural gene for dihydrofolate reductase (dfrA) from the Bacillus subtilis 168 chromosome has been cloned, along with the thyB gene, on a 4.5-kb insert contained on chimeric plasmid pER1. The presence of the dfrA gene on pER1 was demonstrated by showing that: (i) transformation of Escheric...
Topics
- Bacillus subtilis
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- Escherichia coli
- Folic Acid Antagonists
- Genes
- Genes, Bacterial
- Genotype
- Methotrexate
- Phenotype
- Plasmids
- Tetrahydrofolate Dehydrogenase
- Transformation, Bacterial