Direct PCR sequencing of dystrophin polymorphic CACA alleles after purification to remove shadow bands.

doi:10.1089/dna.1992.11.637

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Article

Direct PCR sequencing of dystrophin polymorphic CACA alleles after purification to remove shadow bands.

DNA and cell biology - 1 Oct 1992

Murray V, Monchawin C, England P R, Neilan B A, Leigh D, McDonald B L

Abstract excerpt

A method is described that allows the sequencing of polymerase chain reaction (PCR) products containing CACA repeats. The method was tested using a DNA polymorphism that exists at the 3' end of the dystrophin gene. This polymorphism consists of a variation in the length of a CACA dinucleotide rep...

Topics

Alleles
Artifacts
Autoradiography
Base Sequence
DNA
Dystrophin
Humans
Molecular Sequence Data
Mouth Mucosa
Polymerase Chain Reaction
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid

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