Article
Separation of alveolar macrophages and dendritic cells via autofluorescence: phenotypical and functional characterization.
Journal of leukocyte biology - 1 May 1993
Havenith C E, Breedijk A J, van Miert P P, Blijleven N, Calame W, Beelen R H, Hoefsmit E C
Abstract excerpt
In a previous study we demonstrated that an increase of monocytes and dendritic cells (MDC) was In the current study, the bright autofluorescence of alveolar macrophages (AMs) was used to separate them efficiently from the MDC. Sorting of freshly isolated BAL cells resulted in a high-autofluorescent fraction, consisting predominantly of AMs, and a low-autofluorescent fraction containing the MDC, lymphocytes, and...
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