Article
Murine respiratory tract dendritic cells: isolation, phenotyping and functional studies.
Journal of immunological methods - 1 Apr 2004
Calder Claudia J, Liversidge J, Dick Andrew D
Abstract excerpt
Respiratory tract dendritic cells (RTDC) form a contiguous subepithelial network within the nasorespiratory tract bridging innate and acquired immunity and have been implicated in nasal mucosal tolerance induction. Discrepancies exist between isolation techniques with respect to phenotype and function of RTDC. Therefore, the aim of this study was to modify previous methods to provide a consistent isolation method...
Topics
- Animals
- Antigen Presentation
- Cell Separation
- Cells, Cultured
- Coculture Techniques
- Cytokines
- Dendritic Cells
- Enzyme-Linked Immunosorbent Assay
- Female
- Flow Cytometry
- Immunohistochemistry
