Article
High-frequency transformation of a methylotrophic yeast, Candida boidinii, with autonomously replicating plasmids which are also functional in Saccharomyces cerevisiae.
Journal of bacteriology - 1 Jun 1993
Sakai Y, Goh T K, Tani Y
Abstract excerpt
We have developed a transformation system which uses autonomous replicating plasmids for a methylotrophic yeast, Candida boidinii. Two autonomous replication sequences, CARS1 and CARS2, were newly cloned from the genome of C. boidinii. Plasmids having both a CARS fragment and the C. boidinii URA3...
Topics
- Acetates
- Acetic Acid
- Base Sequence
- Candida
- Cloning, Molecular
- DNA Replication
- Escherichia coli
- Genes, Fungal
- Genetic Vectors
- Methanol
- Molecular Sequence Data
- Orotidine-5'-Phosphate Decarboxylase
- Phenotype
- Plasmids
- Saccharomyces cerevisiae
- Transformation, Genetic