Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Quantitative multiplex sequence analysis of mutational hot spots. Frequency and specificity of mutations induced by a site-specific ethenocytosine in M13 viral DNA.

Biochemistry - 20 Apr 1993

Palejwala V A, Rzepka R W, Simha D, Humayun M Z

Abstract excerpt

We describe an assay for determining the frequency and specificity of mutations occurring at hot spots within a population of DNA molecules. The procedure consists of (a) annealing the DNA population with a labeled oligonucleotide designed to prime DNA synthesis at the mutational hot spot; (b) DNA elongation in the presence of a single dideoxynucleoside triphosphate together with 1-3 deoxynucleoside...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to DNA, Viral
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Complementation Test

Share to Molecular Sequence Data

Share to Mutagenesis, Insertional

Share to Mutation

Share to Transfection

Source

Identifiers

PMID: 8471617
DOI: 10.1021/bi00066a036

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Quantitative multiplex sequence analysis of mutational hot spots. Frequency and specificity of mutations induced by a site-specific ethenocytosine in M13 viral DNA.PMID 8471617Read ↗

Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells.Semantic proximity Read ↗

Next-generation sequencing reveals the biological significance of the N(2),3-ethenoguanine lesion in vivo.Semantic proximity Read ↗

Replication across Regioisomeric Ethylated Thymidine Lesions by Purified DNA PolymerasesSemantic proximity Read ↗

Oxidized, deaminated cytosines are a source of C --> T transitions in vivo.Semantic proximity Read ↗

Miscoding by the exocyclic and related DNA adducts 3,N4-etheno-2'-deoxycytidine, 3,N4-ethano-2'-deoxycytidine, and 3-(2-hydroxyethyl)-2'-deoxyuridine.Semantic proximity Read ↗

Altered UV resistance and UV mutational spectrum in repair-proficient murine fibroblasts expressing endonuclease V.Semantic proximity Read ↗

Genetic effects of exocyclic DNA adducts in vivo: heritable genetic damage in comparison with loss of heterozygosity in somatic cells.Semantic proximity Read ↗

Synthesis of 3,N4-etheno, 3,N4-ethano, and 3-(2-hydroxyethyl) derivatives of 2'-deoxycytidine and their incorporation into oligomeric DNA.Semantic proximity Read ↗

Inhibition of DNA replication fork progression and mutagenic potential of 1, N6-ethenoadenine and 8-oxoguanine in human cell extractsSemantic proximity Read ↗

Genetic and phenotypic changes to Venezuelan equine encephalitis virus following treatment with β-D-N4-hydroxycytidine, an RNA mutagen.Semantic proximity Read ↗

1,N6-ethenodeoxyadenosine, a DNA adduct highly mutagenic in mammalian cells.Semantic proximity Read ↗

Etheno-adduct-forming chemicals: from mutagenicity testing to tumor mutation spectra.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.