Article
Temperature-sensitive amber suppression of ompF'-'lacZ fused gene expression in a supE mutant of Escherichia coli K12.
FEMS microbiology letters - 15 Feb 1993
Kuriki Y
Abstract excerpt
In an ompF'-'lacZ fusion system carried by the open reading frame vector pORF1 in a supE mutant of Escherichia coli K12, read-through of an amber codon was decreased at temperatures higher than 40 degrees C. This effect of temperature was dependent on the nucleotide sequence surrounding the amber...
Topics
- Bacterial Outer Membrane Proteins
- Base Sequence
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genes, Suppressor
- Genes, Synthetic
- Molecular Sequence Data
- Mutation
- Recombinant Fusion Proteins
- Suppression, Genetic
- Temperature
- beta-Galactosidase