OmpF assembly mutants of Escherichia coli K-12: isolation, characterization, and suppressor analysis.

doi:10.1128/jb.175.16.5049-5056.1993

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Article

OmpF assembly mutants of Escherichia coli K-12: isolation, characterization, and suppressor analysis.

Journal of bacteriology - 1 Aug 1993

Misra R

Abstract excerpt

This paper describes a novel genetic method used to isolate mutations that alter proper assembly of OmpF in the outer membrane. The thermolabile nature of assembly intermediates allowed selection of temperature-sensitive mutations within the ompF gene. A variant allele of ompF (ompF-Dex) was used...

Topics

Alleles
Bacterial Outer Membrane Proteins
Cell Membrane
Chromosome Mapping
Codon
Escherichia coli
Mutation
Phenotype
Protein Folding
Suppression, Genetic

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