Article
Unfolded HLA class I alpha chains and their use in an assay of HLA class-I-peptide binding.
Human immunology - 1 Feb 1993
Tanigaki N, Fruci D, Chersi A, Butler R H
Abstract excerpt
Unfolded HLA class I alpha chains were isolated from B-cell lysates by alkaline denaturation and subsequent gel filtration and used for the detection of HLA class-I-peptide binding. Binding to specific peptides in the presence of excess beta 2-microglobulin induced the unfolded alpha chains to re...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- B-Lymphocytes
- Cell Line
- Cells, Cultured
- Evaluation Studies as Topic
- Histocompatibility Antigens Class I
- Humans
- Molecular Sequence Data
- Peptides
- Protein Binding
- Protein Conformation
- Protein Folding
- Rabbits
- Radioimmunoassay
- beta 2-Microglobulin