Two structural states of complexes of peptide and class II major histocompatibility complex revealed by photoaffinity-labeled peptides.

Abstract excerpt

The complex of the murine class II histocompatibility molecules I-A(k) with high affinity binding peptides were resistant to denaturation when examined by SDS-polyacrylamide gel electrophoresis at various pH levels. In contrast, complexes made with low affinity binding peptides were highly sensitive to denaturation by SDS. This effect was more pronounced at low pH. Placing a photoactivatable probe at the amino...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Antigen Presentation
• Share to Cross-Linking Reagents