Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis.

Abstract excerpt

We have purified a minor extracellular serine protease from a strain of Bacillus subtilis bearing null mutations in five extracellular protease genes: apr, npr, epr, bpr, and mpr (A. Sloma, C. Rudolph, G. Rufo, Jr., B. Sullivan, K. Theriault, D. Ally, and J. Pero, J. Bacteriol. 172:1024-1029, 1990). During purification, this novel protease (Vpr) was found bound in a complex in the void volume after gel filtration...

Topics

• Amino Acid Sequence
• Bacillus subtilis
• Base Sequence
• Cloning, Molecular
• DNA, Bacterial
• Electrophoresis, Polyacrylamide Gel