Article
Thiamin-triphosphate-synthesizing activity of mutant cytosolic adenylate kinases: significance of Arg-128 for substrate specificity.
Biochimica et biophysica acta - 13 Feb 1993
Shioda T, Yasuda S, Yamada K, Yamada M, Nakazawa A, Kawasaki T
Abstract excerpt
The thiamin triphosphate (TTP)-synthesizing activity and the ATP-synthesizing activity of two mutant enzymes of chicken cytosolic adenylate kinase whose Arg-128 was substituted by Trp (cAK1(Trp)) or Ala (cAK1(Ala)) were compared to those of the wild-type enzyme. The TTP-synthesizing activity of both the mutant enzymes was higher due to higher affinity to thiamin diphosphate (cAK1(Trp)) or a larger Vmax...
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