Reversible ATP-dependent transition between two forms of human cytosolic thymidine kinase with different enzymatic properties

Abstract excerpt

Human cytosolic thymidine kinase, subunit molecular mass about 24 kDa, is a tetramer in the presence of ATP but a dimer in the presence of thymidine or without substrates. The pure, substrate-free enzyme showed complex, non-hyperbolic thymidine substrate kinetics with an apparent Km of 15 microM. Incubation with ATP at 4 degrees C induced a time-dependent transition to an enzyme form with hyperbolic kinetics and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chronic Lymphocytic Leukemia Research
• Share to Growth Hormone and Insulin-like Growth Factors
• Share to Metabolism, Diabetes, and Cancer