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Article

Intermolecular phosphotransfer is crucial for efficient catalytic activity of nucleoside diphosphate kinase.

The Biochemical journal - 15 Sept 2010

Dar Haider Hussain, Chakraborti Pradip K

Abstract excerpt

NDK (nucleoside diphosphate kinase) is primarily involved in maintaining cellular nucleotide pools in both prokaryotes and eukaryotes. We cloned ndk from Salmonella typhimurium and expressed it in Escherichia coli as a histidine-tagged protein. The Ni-NTA (Ni(2+)-nitrilotriacetate)-purified protein (sNDK) was found to be tetrameric with a monomeric unit molecular mass of approximately 18 kDa. The sNDK exhibited...

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Identifiers

PMID: 20575762
DOI: 10.1042/BJ20100026

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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