In-vitro recombination in rad and rnc mutants of Saccharomyces cerevisiae.

doi:10.1007/BF00336741

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Article

In-vitro recombination in rad and rnc mutants of Saccharomyces cerevisiae.

Current genetics - 1 Jan 1993

Moore P D, Simon J R, Wallace L J, Chow T Y

Abstract excerpt

Extracts of S. cerevisiae cells can catalyze homologous recombination between plasmids in vitro. Extracts prepared from rad50, rad52 or rad54 disruption mutants all have reduced recombinational activity compared to wild-type. The rad52 and rad54 extracts are more impaired in the recombination of...

Topics

Deoxyribonucleases
Fungal Proteins
Genes, Fungal
Mutation
Plasmids
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
tRNA Methyltransferases

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