Article
In-vitro recombination in rad and rnc mutants of Saccharomyces cerevisiae.
Current genetics - 1 Jan 1993
Moore P D, Simon J R, Wallace L J, Chow T Y
Abstract excerpt
Extracts of S. cerevisiae cells can catalyze homologous recombination between plasmids in vitro. Extracts prepared from rad50, rad52 or rad54 disruption mutants all have reduced recombinational activity compared to wild-type. The rad52 and rad54 extracts are more impaired in the recombination of plasmids containing double-strand breaks than of intact plasmids, whereas rad50 extracts are deficient equally for both...
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