Heterologous expression and characterization of wild-type and mutant forms of a 26 kDa endochitinase from barley (Hordeum vulgare L.).

doi:10.1042/bj3220815

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Article

Heterologous expression and characterization of wild-type and mutant forms of a 26 kDa endochitinase from barley (Hordeum vulgare L.).

The Biochemical journal - 15 Mar 1997

Andersen M D, Jensen A, Robertus J D, Leah R, Skriver K

Abstract excerpt

To investigate structure-function relationships in plant chitinases, we have developed a heterologous expression system for the 26 kDa endochitinase from Hordeum vulgare L. (barley). Escherichia coli cells harbouring the gene in a T7 RNA polymerase-based expression vector synthesized completely i...

Topics

Chitinases
DNA, Complementary
Gene Expression Regulation, Plant
Hordeum
Mutagenesis, Site-Directed
Mutation
Recombinant Proteins
Structure-Activity Relationship

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