Improvement of chitinase Pachi with nematicidal activities by random mutagenesis.

Abstract excerpt

Chitinase, an enzyme that can degrade the main compositions of insect intestine and cuticle, has been used in the bio-control field. Our previous work has reported the chitinase Pachi with nematicidal activity (Caenorhabditis elegans). In the present study, to improve the chitinolytic and nematicidal activities of Pachi, a random mutant library was constructed by error-prone PCR and screened by bacteriophage...

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• Caenorhabditis elegans
• Chitinases
• Hydrogen-Ion Concentration
• Metals
• Models, Molecular