Article
Use of an antisense oligonucleotide to inhibit expression of a mutated human procollagen gene (COL1A1) in transfected mouse 3T3 cells.
Biochemistry - 12 Jan 1993
Colige A, Sokolov B P, Nugent P, Baserga R, Prockop D J
Abstract excerpt
A series of antisense oligonucleotides were developed to inhibit specifically expression of a mutated exogenous gene for collagen without inhibiting expression of an endogenous gene for the same protein. The test system consisted of mouse NIH 3T3 cells that were stably transfected with an interna...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Blotting, Western
- Exons
- Fibronectins
- Gene Expression
- Humans
- Introns
- Kinetics
- Mice
- Molecular Sequence Data
- Mutation
- Oligonucleotides, Antisense
- Phosphatidylethanolamines
- Polymerase Chain Reaction
- Procollagen
- RNA, Messenger